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M-NFS-60
M-NFS-60
規(guī)格:
價(jià)格:
編號(hào):B165179
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
M-NFS-60
商品貨號(hào)
B165179
Organism
Mus musculus, mouse
Cell Type
virus induced
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
myelogenous leukemia
Applications
This cell line is a suitable transfection host.
Derivation
The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus.
Comments
The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF).
The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • 62 ng/ml human recombinant macrophage colony stimulating factor (M-CSF)
  • fetal bovine serum to a final concentration of 10%.
    • Subculturing
    Cultures can be maintained by addition or replacement of fresh medium. Subculture every two days at 2.5 X 104 viable cells/mL.
    Medium Renewal: Add fresh medium at the time of subculture
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37°C
    Name of Depositor
    JF Weaver, A Creasey
    Deposited As
    Mus musculus
    References

    Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710

    Weinstein Y, et al. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc. Natl. Acad. Sci. USA 83: 5010-5014, 1986. PubMed: 3014527

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