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MMA
MMA
規格:
價格:
編號:B165175
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MMA
商品貨號
B165175
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with human adherent mononuclear cells and granulocytes.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human monocyte
Cellular Products
immunoglobulin; monoclonal antibody; against human monocyte
Comments
Animals were immunized with the U-937 histiocytic cell line (ATCC CRL-1593).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with human adherent mononuclear cells and granulocytes.
The antibody does not react with circulating T cells, B cells, erythrocytes or platelets.
The antibody is cytotoxic.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM; kappa light chain
Name of Depositor
JF Kearney, H Kubagawa
Deposited As
mouse (B cell); mouse (myeloma)
References

Hanjan SN, et al. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin. Immunol. Immunopathol. 23: 172-188, 1982. PubMed: 6179666

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