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MLE 12

規格:

價格:

編號:B165163

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

MLE 12

商品貨號

B165163

Organism

Mus musculus, transgenic, mouse, transgenic

Tissue

lung

培養基

Cell Type

epithelial; SV40 transformed

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

2 [Cells contain Papovavirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

5 month old

Gender

female

Strain

FVB/N

Storage Conditions

liquid nitrogen vapor phase

Derivation

This line was established in 1992 by Kathryn A. Wikenheiser from pulmonary tumors in a mouse transgenic for the SV40 large T antigen under the control of the promoter region of the human surfactant protein C gene.

Clinical Data

female

5 months

Genes Expressed

lung surfactant proteins B and C (SP-B, SP-C)

Tumorigenic

Yes

Effects

Yes, tumors were generated within 6 to 9 months in nude mice

Comments

The cells express the mRNA for large T antigen.

Lung surfactant proteins B and C were detected.

The cells secrete phospholipids in response to phorbol esters and ATP but not in response to forskolin.

Complete Growth Medium

HITES medium supplemented with 2% fetal bovine serum

Dulbecco's medium : Ham's F12, 50:50 mix (ATCC^® 30-2006)
Insulin 0.005 mg/ml
Transferrin 0.01 mg/ml
Sodium selenite 30 nM
Hydrocortisone 10 nM
β-estradiol 10 nM
HEPES 10 mM
L-glutamine (ATCC^® 30-2214) 2 mM (in addition to that in the base medium)
Fetal bovine serum (ATCC^® 30-2020) 2%

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended

Medium Renewal: Twice per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Population Doubling Time

7 to 9 hrs

Name of Depositor

JA Whitsett

Deposited As

mouse, transgenic

Year of Origin

1992

References

Wikenheiser KA, et al. Simian virus 40 large T antigen directed by transcriptional elements of the human surfactant protein C gene produces pulmonary adenocarcinomas in transgenic mice. Cancer Res. 52: 5342-5352, 1992. PubMed: 1394139

Wikenheiser KA, et al. Production of immortalized distal respiratory epithelial cell lines from surfactant protein C/simian virus 40 large tumor antigen transgenic mice. Proc. Natl. Acad. Sci. USA 90: 11029-11033, 1993. PubMed: 8248207

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