Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The cells were transfected with the pRSV-T plasmid (an SV40 ori- construct containing the SV40 early region and the Rous sarcoma virus long terminal repeat) and cloned.
Mesothelial cells were isolated from pleural fluids obtained from non-cancerous individuals.
The cells stain positively for vimentin, keratins and SV40 T antigen.
To make the complete growth medium, add the following components to the base medium:
10% fetal bovine serum (final conc.)
3.3 nM epidermal growth factor (EGF) (final conc.) (do not filter).
400 nM hydrocortisone (final conc.)
870 nM zinc-free bovine insulin (final conc.)
20 mM HEPES (final conc.)
The trace elements at the following final concentrations:
This medium is formulated for use with a 5% CO2 in air atmosphere.
ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020 (500ml) and ATCC Catalog No. 30-2021 (100ml).
Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989
Lechner JF, et al. Asbestos-associated chromosomal changes in human mesothelial cells. Proc. Natl. Acad. Sci. USA 82: 3884-3888, 1985. PubMed: 2987952
