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MES-SA

規格:

價格:

編號:B165145

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

MES-SA

商品貨號

B165145

Organism

Homo sapiens, human

Tissue

uterus

培養基

Cell Type

Epithelial

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

uterine sarcoma

Age

56 years

Gender

female

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

This is a hypo-diploid human cell line with the modal chromosome number of 45 in 48% of cells examined. The rate of polyploidy was 2.7%. There were five or six marker chromosomes present in each cell. Whereas t(5q6p), 10q+, and 14q+ markers were common in all cells examined, the chromosome combination of either double copy N21 lacking M5 (66.7%) or t(21qter--->C--->?)(M5)/single copy N21/minute metacentric (33.3%) distinguishes the two coexisting clones. Both X chromosomes were normal.

Derivation

The MES-SA cell line was established from a surgical tumor specimen obtained at the time of hysterectomy.

Initially, the cells were grown in soft agar, and later they were transferred to multiwell plates.

Clinical Data

56 years

Caucasian

female

Tumorigenic

Yes

Effects

Yes, readily form colonies in soft agar.

Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Comments

The tumor was described as a poorly differentiated uterine sarcoma.

The nonepithelial origin of the cells was supported by ultrastructural studies and the absence of staining for mucin.

The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin. They are resistant to vinblastine, dacarbazine, cisplatin, melphalan, vincristine, methotrexate and etoposide.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 mL double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Note: Newborn calf serum may be substituted for fetal bovine serum.

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 11

D13S317: 11,13

D16S539: 11,12

D5S818: 13

D7S820: 7,11

THO1: 6

TPOX: 8,11

vWA: 18

Population Doubling Time

22 to 24 hrs

Name of Depositor

BI Sikic

Deposited As

Homo sapiens

Year of Origin

1980

References

Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344

B165146：MES-SA/MX2

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