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MDA-MB-468

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編號:B165129

品牌:Mingzhoubio

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產品名稱

MDA-MB-468

商品貨號

B165129

Organism

Homo sapiens, human

Tissue

mammary gland/breast; derived from metastatic site: pleural effusion

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

adenocarcinoma

Age

51 years

Gender

female

Ethnicity

Black

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 64; range = 60 to 67.
The cell line is aneuploid human, presumably female (X, abnormal X) with most chromosome counts in the hypotriploid range.; Normal chromosomes X, N2, N3, N7, N8, N10, and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line.; A normal chromosome N1 (or two) is identified in each karyotype, but, in addition, regions of chromosome N1 are also present in five different marker chromosomes.; Variation is evident in the normal and marker chromosome copy number from karyotype to karyotype.

Images

Derivation

The MDA-MB-468 cell line was isolated in 1977 by R. Cailleau, et al., from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast.

Clinical Data

51 years

Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype.

Black

female

Antigen Expression

Blood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4 (patient)

Receptor Expression

epidermal growth factor (EGF); transforming growth factor alpha (TGF alpha)

Tumorigenic

Yes

Effects

Yes, in nude mice inoculated subcutaneously with 10(7) cells.

(Tumors developed within 21 days at 100% frequency (5/5).)

Comments

Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. EGF receptor is present at 1 X 10⁶ per cell.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Subculturing

Volumes are given for a 75 cm²flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 100%

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 12

D13S317: 12

D16S539: 9

D5S818: 12

D7S820: 8

THO1: 7

TPOX: 8,9

vWA: 18

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, A

GLO-I, 1-2

Me-2, 1-2

PGM1, 1

PGM3, 2

Name of Depositor

R Cailleau

Deposited As

Homo sapiens

Year of Origin

1977

References

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Pathak S, et al. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line. J. Natl. Cancer Inst. 62: 263-271, 1979. PubMed: 283262

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845

Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006

Avila MA, et al. Quercetin mediates the down-regulation of mutant p53 in the human breast cancer cell line MDA-MB468. Cancer Res. 54: 2424-2428, 1994. PubMed: 8162591

Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764

Zamora-Leon SP, et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996. PubMed: 8700847

Tumors developed within 21 days at 100% frequency (5/5).

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