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MDA-MB-330
MDA-MB-330
規格:
價格:
編號:B165124
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MDA-MB-330
商品貨號
B165124
Organism
Homo sapiens, human
Tissue
mammary gland; breast; Derived from metastatic site: pleural effusion
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Infiltrating lobular carcinoma
Age
43 years
Gender
female
Ethnicity
Caucasian
Applications
The population exhibited a mixed phenotype when examined by immunofluorescence staining for tubulin, with some cells (type I) having extensive arrays and others (type II) a more diffuse reaction pattern.
Karyotype
modal number = 64 with one marker chromosome
Images
Clinical Data
female
Caucasian
43 years
Antigen Expression
Blood Type A; Rh+
Receptor Expression
epidermal growth factor (EGF)
Cellular Products
tubulin; actin
Tumorigenic
No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments
The population exhibited a mixed phenotype when examined by immunofluorescence staining for tubulin, with some cells (type I) having extensive arrays and others (type II) a more diffuse reaction pattern.
Most cells of the line stained positively for actin.
This line grows very slowly (doubling time is 10 to 20 days) and never becomes confluent.
Complete Growth Medium
These cells are grown in Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with:
  • 30 ng/ ml human recombinant EGF
  • 0.016 mg/ml human recombinant insulin
  • 2mM glutathione
  • 20% fetal bovine serum
Do not filter EGF thru 0.22 micron filter.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Once per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and let the culture sit at 37C until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 100%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 9,12
D5S818: 13
D7S820: 10,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14,16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1
Population Doubling Time
240 to 480 hrs
Name of Depositor
R Cailleau
Deposited As
Homo sapiens
References

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

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