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MDA-MB-175-VII

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價格:

編號:B165122

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

MDA-MB-175-VII

商品貨號

B165122

Organism

Homo sapiens, human

Tissue

mammary gland; breast/duct; derived from metastatic site: pleural effusion

培養基

Cell Type

epithelial

Product Format

frozen

Morphology

epithelial

Culture Properties

loosely adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

ductal carcinoma

Age

56 years

Gender

female

Ethnicity

Black

Storage Conditions

liquid nitrogen vapor phase

Karyotype

model number = 84; range = 82 to 89. The cell line is aneuploid female (XX/XXX/XXXX), with chromosome counts in the hypertetraploid to near-tetraploid range. All normal chromosomes are represented by at least one copy, with most chromosomes having three or four copies present per karyotype. Five marker chromosomes are found: del(1)p35), der(7)t(1;7)(q11;q32), unknown, t(2q;?), unknown isochromosome. Several are present in multiple copy. Alterations in the q arm of chromosome N1 are in accordance with the report by M.J. Siciliano, et al., Cancer Res. 39: 919, 1979.

Images

Derivation

This is one of a series of breast tumor lines (ATCC HTB-23 through HTB-26) isolated from pleural effusions and characterized by R. Cailleau and associates in 1973.

Clinical Data

56 years

Black

female

Tumorigenic

Yes

Effects

Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 100%

STR Profile

Amelogenin: X

CSF1PO: 7,8

D13S317: 11,12

D16S539: 11,13

D5S818: 11

D7S820: 11,12

THO1: 7

TPOX: 6,8

vWA: 15,16

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

PGM1, 2

PGM3, 1-2

Name of Depositor

R Cailleau

Deposited As

Homo sapiens

References

Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

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