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MD
MD
規(guī)格:
價(jià)格:
編號(hào):B165116
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
MD
商品貨號(hào)
B165116
Organism
Homo sapiens, human
Tissue
spleen
培養(yǎng)基
Cell Type
monocyte/macrophage
Product Format
frozen
Morphology
monocyte/macrophage
Culture Properties
loosely adherent, multicell aggregates
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
The MD cell line was established from cryopreserved human spleen cells.
Comments
Epstein-Barr virus has been detected in this cell line.
Complete Growth Medium
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 X 105 viable cells/mL. A maximum of 1 to 2 X 106 is obtainable.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11,15
D5S818: 11,13
D7S820: 10,11
THO1: 8
TPOX: 8,11
vWA: 16,17
Name of Depositor
E.I. du Pont de Nemours & Co.
Deposited As
Homo sapiens
U.S. Patent Number
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995

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