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MC-SV-HUC T-2
MC-SV-HUC T-2
規(guī)格:
價格:
編號:B165115
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
MC-SV-HUC T-2
商品貨號
B165115
Organism
Homo sapiens, human
Tissue
ureter, immortalized epithelial SV40-transformed
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 Cells contain polyomavirus DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
11 years
Gender
male
Applications
The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Clinical Data
male
Genes Expressed
uroepithelial keratins
Cellular Products
uroepithelial keratins
Tumorigenic
Yes
Effects
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Comments
The cells were initially transformed with SV40 virus, and later treated with 3-methylcholanthrene (MCA).
While the parental line (SV-HUC-1, see ATCC CRL-9520) was not tumorigenic and had a balanced chromosome composition, the MCA treated line became tumorigenic and aneuploid.
The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative.
Stress (such as chemical exposure) could possibly activate the virus.
Complete Growth Medium
Ham's F12 medium, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
Wisconsin Alumni Res. Fndn.
U.S. Patent Number
References

Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990

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