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MC-IXC
MC-IXC
規格:
價格:
編號:B165114
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MC-IXC
商品貨號
B165114
Organism
Homo sapiens, human
Tissue
brain; derived from metastatic site: supra-orbital area
培養基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
neuroblastoma
Age
14 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
doubles minutes (DM) are prevalent
Derivation
MC-IXC is a twice cloned subline of the neuroepithelioma cell line SK-N-MC (see ATCC HTB-10) which was established in September of 1971 from a metastatic tumor mass.
Clinical Data
14 years
Caucasian
female
Antigen Expression
Blood Type O; Rh+
Comments

MC-IXC is a twice cloned subline of the neuroepithelioma cell line SK-N-MC (see ATCC HTB-10) which was established in September of 1971 from a metastatic tumor mass.

The choline acetyltransferase activity of MC-IXC was approximately four times higher than the parental line but MC-IXC was negative for dopamine beta hydroxylase activity as was the parental line.

MC-IXC cells have a reported saturation density greater than 1 X 106 cells/cm2.

The cells are fibroblast-like and grow to form a dense monolayer.

Floating cells are nonviable.
Complete Growth Medium
The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C

Subculture Ratio: 1:10 to 1:20
Medium Renewal: Every 4 to 7 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11
D7S820: 8
THO1: 9.3
TPOX: 9,11
vWA: 17,18
Population Doubling Time
36 hrs
Name of Depositor
JL Biedler
Deposited As
Homo sapiens
Year of Origin
1971
References

Biedler JL, et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 33: 2643-2652, 1973. PubMed: 4748425

Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704

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