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MC116

規格:

價格:

編號:B165099

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

MC116

商品貨號

B165099

Organism

Homo sapiens, human

Tissue

ascites

培養基

Cell Type

B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

undifferentiated lymphoma

Gender

male

Karyotype

45,XO/46,XY, dup1(q21-32), t(8;14)(8pter --> 8q23 :: 14q32 --> 14qter ; 14pter --> 14q32 :: 8q23 --> 8qter), del10(a23)

Clinical Data

male

Antigen Expression

HLA A1, A3, B8, Bw35

Genes Expressed

immunoglobulin (surface and secreted)

Cellular Products

immunoglobulin (surface and secreted)

Comments

The cells are EBNA negative, negative for Fc receptors and negative for complement receptors.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing

Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 3 X 10⁵ viable cells/mL and maintain at or below 3 X 10⁶ cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation

culture medium 95%; DMSO, 5%

Isotype

IgM; lambda light chain

Population Doubling Time

26 to 28 hrs

Name of Depositor

IT Magrath

Deposited As

Homo sapiens

References

Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721

Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722

Cross References

Nucleotide (GenBank) : U07991 Human lymphoma immunoglobulin lambda light chain mRNA, partial cds.

Nucleotide (GenBank) : U07988 Human lymphoma immunoglobulin IgM heavy chain Fd fragment mRNA, partial cds.

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