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MC/9

規格:

價格:

編號:B165096

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

MC/9

商品貨號

B165096

Organism

Mus musculus, mouse

Tissue

liver

培養基

MCM2268：MC/9細胞專用培養基

Cell Type

mast cell

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

13 days

Strain

(B6 X A/J)F1

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

This is a cloned mast cell line derived from the fetal liver of a (B6 X A/J)F1 mouse.

Receptor Expression

FcERI (Fc of IgE)

Genes Expressed

histamine; leukotrienes

Cellular Products

histamine; leukotrienes

Comments

The cells can be sensitized to specific antigens by incubating them with IgE having the desired antigenic specificity.

The cells so coated will release soluble mediators (such as histamine) in response to exposure to the antigen (allergen).

Complete Growth Medium

Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine adjusted to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and supplemented with 2 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10% Rat T-STIM (Becton Dickenson Catalog No. 354115) and 10% fetal bovine serum.

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 5 X 10⁵ viable cells/ml. Maintain cell density between 2 X 10⁵ and 2 X 10⁶ viable cells/mL. Do not allow the cell density to exceed 2 X 10⁶ cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Culture Conditions

Temperature: 37°C

Name of Depositor

Dana Farber Cancer Inst.

Deposited As

Mus musculus

U.S. Patent Number

4,559,310

References

Cantor HI, Nabel G. Assay methods and systems utilizing mast cell clones. US Patent 4,559,310 dated Dec 17 1985

Nabel G, et al. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell 23: 19-28, 1981. PubMed: 6971154

Galli SJ, et al. Mast cell clones: a model for the analysis of cellular maturation. J. Cell Biol. 95: 435-444, 1982. PubMed: 6216259

Lewin I, et al. Aggregation of the FcepisilonRI in mast cells induces the synthesis of fos-interacting protein and increases its DNA binding activity: the dependence on protein kinase c-beta. J. Biol. Chem. 271: 1514-1519, 1996. PubMed: 8576146

Ishizuka T, et al. Aggregation of the FceRI on mast cells stimulates c-Jun amino-terminal kinase activity. J. Biol. Chem. 271: 12762-12766, 1996. PubMed: 8662803

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