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Malme-3M

規(guī)格:

價(jià)格:

編號(hào):B165075

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

Malme-3M

商品貨號(hào)

B165075

Organism

Homo sapiens, human

Tissue

malignant melanoma; Derived from metastatic site: lung

培養(yǎng)基

Cell Type

fibroblast

Product Format

frozen

Morphology

mixed

Culture Properties

mixed adherent-suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Malignant melanoma

Age

43 years

Gender

male

Ethnicity

Caucasian

Applications

This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. The two cell lines provide tumor and normal counterparts for comparative in vitro melanoma studies.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 88; range = 76 to 93
This is a hypotetraploid human cell line with the modal chromosome number of 88 occurring in 17% of cells. Cells having 82 to 87 chromosome counts were also abundant. Six marker chromosomes were common to most cells, including i(1q), i(6p), i(7q), t(4qter--4pter::?::4q11--4qter) and two others. Except for the paired i(7q), all were single. Two copies of normal X and Y chromosomes were detected in most cells. Normal chromosomes N3 and N17 had five copies in most cells.

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Derivation

This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh.

This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. -

Clinical Data

Caucasian

male

This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast.

43 years

Antigen Expression

Antigen expression: HLA A2, Aw30, B13, B40(+/-), DRw7

Tumorigenic

Yes

Effects

Yes, in nude mice; forms pigmented malignant melanoma

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing

Remove to a centrifuge tube.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 x g for 5 to 10 minutes.
Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Two to three times weekly

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

STR Profile

Amelogenin: X,Y

CSF1PO: 12

D13S317: 8,13

D16S539: 9,12

D5S818: 11

D7S820: 9,12

THO1: 8

TPOX: 8,9

vWA: 15,16

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1

Name of Depositor

J Fogh

Deposited As

Homo sapiens

References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

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