最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > M3WT8 B165054
M3WT8
M3WT8
規格:
價格:
編號:B165054
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
M3WT8
商品貨號
B165054
Organism
Cricetulus griseus, hamster, Chinese
Product Format
frozen
培養基
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender
female
Applications
The M3WT8 cell line was derived by transfecting CHO-K1 cells (ATCC CCL-61) with the pSVL expression vector containing the gene for the rat m3 muscarinic acetylcholine receptor.
Storage Conditions
liquid nitrogen vapor phase
Derivation
The M3WT8 cell line was derived by transfecting CHO-K1 cells (ATCC CCL-61) with the pSVL expression vector containing the gene for the rat m3 muscarinic acetylcholine receptor.
Clinical Data
female
Receptor Expression
acetylcholine, muscarinic m3
Comments
The M3WT8 cell line was derived by transfecting CHO-K1 cells (ATCC CCL-61) with the pSVL expression vector containing the gene for the rat m3 muscarinic acetylcholine receptor.
The resulting cells were co-transfected with pMSVNeo and selected for growth in medium containing 0.5 mg/ml G418.
M3WT8 cells are reported to express 75 fmol of receptor protein per mg of membrane protein.
Complete Growth Medium
Ham's F12 medium with 0.05 to 0.1 mg/ml G418, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature (or incubate at 37C) until the cells detach (usually 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: At the time of subcultivation
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor
CM Fraser
Deposited As
Cricetulus griseus
References

Buck MA, Fraser CM. Muscarinic acetylcholine receptor subtypes which selectively couple to phospholipase C: pharmacological and biochemical properties. Biochem. Biophys. Res. Commun. 173: 666-672, 1990. PubMed: 2175603

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479