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M3/84.6.34

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價格:

編號:B165051

品牌:Mingzhoubio

標準菌株

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DNA

RNA

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凍干粉

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產品名稱

M3/84.6.34

商品貨號

B165051

Organism

Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)

Tissue

spleen

培養基

Cell Type

hybridoma: B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

Mac-2 and Mac-3 are present on 69% of macrophages and 0% to 2% of thymocytes.

Tested and found negative for ectromelia virus (mousepox).

Storage Conditions

liquid nitrogen vapor phase

Genes Expressed

Tested and found negative for ectromelia virus (mousepox).

immunoglobulin; monoclonal antibody; against Mac-3 (mouse macrophage antigen, 110000 dalton glycoprotein),Expression of Mac-3 is increased during the differentiation from monocyte to activated peritoneal macrophage.

Cellular Products

immunoglobulin; monoclonal antibody; against Mac-3 (mouse macrophage antigen, 110000 dalton glycoprotein)

Comments

Animals were immunized with detergent solubilized mouse(C57BL/6) macrophage membrane which had been depleted of previously identified antigens with monoclonal immunoadsorbants.

Like Mac-2, the Mac-3 antigen is not expressed on bone marrow cells (Mac-1 is expressed on bone marrow cells).

Also like Mac-2, Mac-3 appears to be expressed on their monocytic line of differentiation at a stage after divergence from the granulocytic series.

Mac-2 and Mac-3 are present on 69% of macrophages and 0% to 2% of thymocytes.

Expression of Mac-3 is increased during the differentiation from monocyte to activated peritoneal macrophage.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Adherent cells can be dislodged by scraping and cultures established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.

Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Isotype

rat IgG1 kappa

Name of Depositor

TA Springer

Deposited As

rat (B cell); mouse (myeloma)

References

Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J. Biol. Chem. 256: 3833-3839, 1981. PubMed: 7217058

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