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M3/38.1.2.8 HL.2
M3/38.1.2.8 HL.2
規格:
價格:
編號:B165050
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
M3/38.1.2.8 HL.2
商品貨號
B165050
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue
spleen
培養基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Mac-2 and Mac-3 are present on 69% of macrophages and 0% to 2% of thymocytes.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
Tested and found negative for ectromelia virus (mousepox).
immunoglobulin; monoclonal antibody; against mouse macrophage antigen (Mac-2, 32000 dalton glycoprotein),Expression of Mac-2 is increased during the differentiation from monocyte to activated peritoneal macrophage.
Cellular Products
immunoglobulin; monoclonal antibody; against mouse macrophage antigen (Mac-2, 32000 dalton glycoprotein)
Comments
Spleen cells were fused with NS-1 myeloma cells.
The Mac-2 antigen is not expressed on bone marrow cells.
Like Mac-3, Mac-2 appears to be expressed on the monocytic line of differentiation at a stage after divergence from the granulocytic series.
Mac-2 and Mac-3 are present on 69% of macrophages and 0% to 2% of thymocytes.
Expression of Mac-2 is increased during the differentiation from monocyte to activated peritoneal macrophage.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Adherent cells can be dislodged by scraping and cultures established by centrifugation with subsequent resuspension at 2 to 4 X 10(5) viable cells/ml.
Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
rat IgG2a kappa
Name of Depositor
TA Springer
Deposited As
rat (B cell); mouse (myeloma)
References

Ho MK, Springer TA. MAC-2, a novel 32,000 Mr mouse macrophage subpopulation-specific antigen defined by monoclonal antibodies. J. Immunol. 128: 1221-1228, 1982. PubMed: 6173426

Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J. Biol. Chem. 256: 3833-3839, 1981. PubMed: 7217058

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