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M17/5.2
M17/5.2
規格:
價格:
編號:B165043
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
M17/5.2
商品貨號
B165043
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue
spleen
培養基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody blocks CTL mediated cytotoxicity, and can be used to precipitate LFA-1 from lysates of CTLs.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse lymphocyte function antigen 1 (LFA-1, CD11a)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse lymphocyte function antigen 1 (LFA-1, CD11a)
Comments
Animals were immunized with mouse cytotoxic T lymphocytes (CTL).
Spleen cells were fused with NS-1 myeloma cells.
The antibody blocks CTL mediated cytotoxicity, and can be used to precipitate LFA-1 from lysates of CTLs.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL.
Interval: Maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Isotype
rat IgG2b kappa
Name of Depositor
TA Springer
Deposited As
rat (B cell); mouse (myeloma)
References

Sanchez-Madrid F, et al. Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell. Immunol. 73: 1-11, 1982. PubMed: 6983917

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