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M1/75.16.4.HLK
M1/75.16.4.HLK
規(guī)格:
價格:
編號:B165039
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
M1/75.16.4.HLK
商品貨號
B165039
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue
spleen
培養(yǎng)基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse heat stable antigen
Cellular Products
immunoglobulin; monoclonal antibody; against mouse heat stable antigen
Comments
Animals were immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody does not lyse thymocytes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Subcultivation Ratio: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
rat IgG2c , IgG2c
Name of Depositor
TA Springer
Deposited As
rat (B cell); mouse (myeloma)
References

Springer T, et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8: 539-551, 1978. PubMed: 81133

Springer TACell -surface differentiation in the mouse: Characterization of Jumping and Lineage antigens using xenogeneic rat monoclonal antibodiesIn: Springer TAMonoclonal AntibodiesNew YorkPlenum Presspp. 185-217, 1980

Milstein C, et al. Monoclonal antibodies and cell surface antigens. Cell Biol. Int. Rep. 3: 1-16, 1979. PubMed: 88272

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