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當(dāng)前位置: 首頁(yè) > ATCC代理 > M/K-1.9 B165028
M/K-1.9
M/K-1.9
規(guī)格:
價(jià)格:
編號(hào):B165028
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
M/K-1.9
商品貨號(hào)
B165028
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine VCAM-1
Cellular Products
immunoglobulin; monoclonal antibody; against murine VCAM-1
Tumorigenic
Yes
Effects
Yes, forms ascites in CB.17.SCID mice
Comments
Animals were immunized with stromal cells from mouse bone marrow.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody blocks adhesion of lymphocyte precursors to bone marrow stromal cells.
The antibody recognizes an antigen believed to be the mouse equivalent of human VCAM-1 that is expressed on bone marrow stromal cells and on inflamed endothelial cells (see also M/K-2.7, ATCC CRL-1909).
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
PW Kincade
Deposited As
rat (B cell); mouse (myeloma)
References

Miyake K, et al. A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture. J. Cell Biol. 114: 557-565, 1991. PubMed: 1713592

Miyake K, et al. Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. J. Exp. Med. 173: 599-607, 1991. PubMed: 1997648

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