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Loucy
Loucy
規格:
價格:
編號:B164999
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Loucy
商品貨號
B164999
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
T lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
T-cell acute lymphoblastic leukemia
Age
38 years
Gender
female
Ethnicity
White
Applications
This cell line may be of value in evaluating the role of t(16;20) in the etiology of T-ALL. It is important for genetic studies.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
45,X,5q-,t(16;20)(p12;q13) RefBen-Bassat H, et al. Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation. Cancer Genet. Cytogenet. 49: 241-248, 1990. PubMed: 2208060
Derivation
The Loucy cell line was established in 1987 from the peripheral blood of a patient with T-cell acute lymphoblastic leukemia (T-ALL) obtained two months prior to her death
Clinical Data
38 years adult
female
White
Antigen Expression
OKT3+, OKT4+, THB4+, J5 +/-, OKT6-, TdT-, and HLA-DR-
Oncogene
p53 + overexpressed; (mutated, GTG --> ATG mutation at codon 272) RefProkocimer M, et al. P53 gene mutation in a T-acute lymphoblastic leukemia cell line (loucy) with t(16:20) and 5q- chromosomal aberrations. Leuk. Lymphoma 29: 607-611, 1998. PubMed: 9643574
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL.

Interval: Maintain cultures at cell concentrations between 3 x 105 and 3 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,13
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 8,9.3
TPOX: 8,9
vWA: 16,17
Population Doubling Time
24 to 36 hrs
Name of Depositor
H Ben-Bassat
Deposited As
human
Year of Origin
November, 1987
References

Ben-Bassat H, et al. Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation. Cancer Genet. Cytogenet. 49: 241-248, 1990. PubMed: 2208060

Prokocimer M, et al. P53 gene mutation in a T-acute lymphoblastic leukemia cell line (loucy) with t(16:20) and 5q- chromosomal aberrations. Leuk. Lymphoma 29: 607-611, 1998. PubMed: 9643574

Ben-Bassat H, et al. Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation. Cancer Genet. Cytogenet. 49: 241-248, 1990. PubMed: 2208060

Prokocimer M, et al. P53 gene mutation in a T-acute lymphoblastic leukemia cell line (loucy) with t(16:20) and 5q- chromosomal aberrations. Leuk. Lymphoma 29: 607-611, 1998. PubMed: 9643574

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