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當(dāng)前位置: 首頁 > ATCC代理 > LL/2 (LLC1) B164982
LL/2 (LLC1)
LL/2 (LLC1)
規(guī)格:
價(jià)格:
編號:B164982
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
LL/2 (LLC1)
商品貨號
B164982
Organism
Mus musculus, mouse
Tissue
lung
培養(yǎng)基
Product Format
frozen
Morphology
rounded - loosely attached or floating
Culture Properties
mixed, adherent and suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Lewis lung carcinoma
Strain
C57BL
Applications
This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.
Antigen Expression
H-2b
Tumorigenic
Yes
Effects
Yes, in C57BL mice
Comments
The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.
The cells are reported to be highly tumorigenic, but weakly metastatic in mice.
The cells form multilayers in flasks without actually becoming confluent.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Population Doubling Time
21 hrs
Name of Depositor
JS Bertram
Deposited As
Mus musculus
References

Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139

Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function. J. Immunol. 163: 5020-5028, 1999. PubMed: 10528207

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