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Lec2 [originally named Pro-5WgaRII6A]
Lec2 [originally named Pro-5WgaRII6A]
規格:
價格:
編號:B164969
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Lec2 [originally named Pro-5WgaRII6A]
商品貨號
B164969
Organism
Cricetulus griseus, hamster, Chinese
Tissue
ovary
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
monolayer or suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender
female
Applications
This cell line exhibits a drastic reduction in the transport of CMP-sialic acid into the Golgi compartment, and may be useful for studying the role of sialic acid in the function and compartmentalization of glycosylated macromolecules.
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin.
Clinical Data
female
Comments
The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).
The population contains Pro+ revertants at high frequency (approximately 1 in 25 cells), and should be cloned if it is desired to use the Pro- marker in complementation studies.
Complete Growth Medium
Alpha minimum essential medium, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, add fresh 0.25% trypsin, let sit for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Name of Depositor
P Stanley
Deposited As
Cricetulus griseus
References

Deutscher SL, et al. Translocation across Golgi vesicle membranes: a CHO glycosylation mutant deficient in CMP-sialic acid transport. Cell 39: 295-299, 1984. PubMed: 6498937

Stanley P, Siminovitch L. Complementation between mutants of CHO cells resistant to a variety of plant lectins. Somatic Cell Genet. 3: 391-405, 1977. PubMed: 601679

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