Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
A minority of cells are Fc receptor positive and an appreciable number of cells are complement receptor 1 positive.
The cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. The cells are not phagocytic for antibody or complement-coated particles; they do not constitutively secrete Interleukin-1.
LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages.
The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Generation of conditioned Medium
LADMAC conditioned medium is made from LADMAC cells (ATCC CRL-2420). LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1.Ref
Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
Sklar MD, et al. Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730
Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
