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La1 [anti-La/SSB]
La1 [anti-La/SSB]
規(guī)格:
價(jià)格:
編號:B164953
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
La1 [anti-La/SSB]
商品貨號
B164953
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for bovine La/SSB, but can be used in an indirect ELISA to detect anti La/SSB antibodies in human sera.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified homogeneous bovine La/SSB.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine La/SSB (autoimmune RNA protein)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine La/SSB (autoimmune RNA protein)
Comments
Animals were immunized with purified homogeneous bovine La/SSB.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for bovine La/SSB, but can be used in an indirect ELISA to detect anti La/SSB antibodies in human sera.
Some cross-reactivity with Ro/SSB is seen in this assay.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 90%; DMSO, 10%
Isotype
IgG
Name of Depositor
University of Oklahoma
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Harley JB. Monoclonal antibodies against autoimmune RNA proteins. US Patent 4,784,942 dated Nov 15 1988

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