Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Unlike the L6 cell line (ATCC CRL-1458) no carcinogen was used to establish the L8 line.
Upon becoming confluent, L8 will fuse to form cross striated multinucleated muscle fibers.
It is recommended that early passages be preserved in liquid nitrogen and that the line be recloned periodically and reselected for progeny that have the ability to fuse.
It is important that the cells be subcultured when the flask is about 60% confluent. The myoblastic population will be depleted if the cultures are allowed to become confluent since most of the cells will fuse into nondividing syncytia. To avoid this, one must subculture before before the cultures become confluent and should reclone periodically and select myoblastic clones.
Richler C, Yaffe D. The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev. Biol. 23: 1-22, 1970. PubMed: 5481965
Yaffe D, Saxel O. A myogenic cell line with altered serum requirements for differentiation. Differentiation 7: 159-166, 1977. PubMed: 558123
