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當(dāng)前位置: 首頁 > ATCC代理 > L-14 [L-14-SCRF 55.1] B164936
L-14 [L-14-SCRF 55.1]
L-14 [L-14-SCRF 55.1]
規(guī)格:
價(jià)格:
編號(hào):B164936
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
L-14 [L-14-SCRF 55.1]
商品貨號(hào)
B164936
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a 72000 dalton HMCV immediate - early perinuclear antigen.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human cytomegalovirus (HMCV) immediate - early 72000 dalton antigen
Cellular Products
immunoglobulin; monoclonal antibody; against human cytomegalovirus (HMCV) immediate - early 72000 dalton antigen
Tumorigenic
Yes
Effects
Yes, forms ascites forms ascites in BALB/c mice
Comments
Animals were immunized with CMV infected human fibroblasts.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a 72000 dalton HMCV immediate - early perinuclear antigen.
The antigen is expressed within three hours of infection with HCMV.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/ml and maintain between 1 X 105 and 1 X 106 cells/ml.
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Isotype
IgG1
Name of Depositor
Scripps Clinic and Research Foundation
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Rice GP, et al. Cytomegalovirus infects human lymphocytes and monocytes: Virus expression is restricted to immediate-early gene products. Proc. Natl. Acad. Sci. USA 81: 6134-6138, 1984. PubMed: 6091137

March CJ, et al. Hybridoma antibody which binds IL-2 receptor. US Patent 4,845,198 dated Jul 4 1989

March CJ, et al. Interleukin-2 receptor. US Patent 4,578,335 dated Mar 25 1986

Oldstone MB, Rice G. Diagnostic system for the detection of cytomegalovirus. US Patent 4,818,678 dated Apr 4 1989

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