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L-132
L-132
規(guī)格:
價格:
編號:B164935
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
L-132
商品貨號
B164935
Organism
Homo sapiens, human
Product Format
frozen
培養(yǎng)基
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 Cells contain Human papillomavirus

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
Derivation
This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
HeLa Markers
Y
Genes Expressed
keratin,The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
keratin
Comments
This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes. Remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor
EV Davis
Deposited As
Homo sapiens
References

Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

Erfle V, Mellert W. Human cell line LC5 and its use. US Patent 5,582,967 dated Dec 10 1996

. . Fed. Proc. 19: 386, 1960.

. . Science 133: 2059, 1961.

Tresnan DB, et al. Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. J. Virol. 70: 8669-8674, 1996. PubMed: 8970993

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