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KLN 205
KLN 205
規格:
價格:
編號:B164918
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
KLN 205
商品貨號
B164918
Organism
Mus musculus, mouse
Tissue
lung
培養基
Product Format
frozen
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
squamous cell carcinoma
Strain
DBA/2
Storage Conditions
lquid nitrogen vapor phase
Images
Cell Micrograph KLN 205, ATCC CRL-1453 cells
Tumorigenic
Yes
Effects
Yes, in DBA/2 and BDF1 mice
Comments
KLN 205 cells form metastatic lesions in lungs after inoculation into mice.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: lquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor
T Kaneko
Deposited As
Mus musculus
References

Kaneko T, LePage GA. Growth characteristics and drug responses of a murine lung carcinoma in vitro and in vivo. Cancer Res. 38: 2084-2090, 1978. PubMed: 418873

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