Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
After the tenth passage, the parental KG-1 cells were cultured in two separate laboratories within the same department under identical conditions.
After 35 passages the cells in one laboratory expressed morphological differences from the parent line. The variant KG-1a was composed of undifferentiated promyeloblasts.
Both populations exhibit many common characteristics. They share a similar doubling time, are negative for EBNA and VCA, express no surface immunoglobulins and exhibit identical HLA and isoenzyme profiles.
In contrast to the parental KG-1 (ATCC CCL-246) the KG-1a population is unresponsive to colony-stimulating factor in soft-agar culture and does not express the Ia-like antigen.
KG-1a cells are resistant to phorbol diester induced macrophage differentiation and proliferation of the cells is unaffected by the presence of phorbol diesters
.The KG-1a cells are morphologically, cytochemically, and functionally less mature than the parental KG-1.
Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Twice per week
Koeffler HP. Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood 62: 709-721, 1983. PubMed: 6192859
Koeffler HP, et al. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1). Blood 56: 265-273, 1980. PubMed: 6967340
Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679
Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022
