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K204
K204
規(guī)格:
價格:
編號:B164899
品牌:Mingzhoubio

標(biāo)準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
K204
商品貨號
B164899
Organism
Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with mouse non k haplotype class I major histocompatibility antigens (H-2).
Derivation
Spleen cells were fused with Y3-Ag.1.2.3 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against H-2 from non k haplotype mice
Cellular Products
immunoglobulin; monoclonal antibody; against H-2 from non k haplotype mice
Comments
Animals were immunized with 3T3-Swiss albino cells (ATCC CCL-92).
Spleen cells were fused with Y3-Ag.1.2.3 myeloma cells.
The antibody reacts with mouse non k haplotype class I major histocompatibility antigens (H-2).
Complete Growth Medium
Culture Medium: RPMI medium 1640 with 0.05 mM 2-mercaptoethanol and 4 mM
L-glutamine, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
M Mage, K Yamada, T Hasegawa
Deposited As
rat (B cell); rat (myeloma)
References

Nardelli B, et al. Polyacrylamide-streptavidin: A novel reagent for simplified construction of soluble multivalent macromolecular conjugates. J. Immunol. Methods 120: 233-239, 1989. PubMed: 2738413

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