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K117
K117
規(guī)格:
價格:
編號:B164898
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
K117
商品貨號
B164898
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Astrocytoma,sarcoma
Strain

Strain:? (BALB/c X C57BL/6)F1 (B cell); BALB/c (myeloma)

Applications
The antibody reacts with the human Thy-1 antigen, a glycoprotein (gp25) of relative molecular weight 25000 daltons.
The antigen is expressed on variety of normal and neoplastic tissues and is present on most sarcomas and tumors of neuroectodermal origin.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Genes Expressed
immunoglobulin; monoclonal antibody; against a human astrocytoma cell line; human Thy-1, gp25 glycoprotein
Cellular Products
immunoglobulin; monoclonal antibody; against a human astrocytoma cell line; human Thy-1, gp25 glycoprotein
Comments
Animals were immunized with the SK-MG-2 human astrocytoma cell line.
The antibody reacts with the human Thy-1 antigen, a glycoprotein (gp25) of relative molecular weight 25000 daltons.
The antigen is expressed on variety of normal and neoplastic tissues and is present on most sarcomas and tumors of neuroectodermal origin.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 92.5%; DMSO, 7.5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Sloan-Kettering Institute for Cancer Research
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991

Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. PubMed: 2877731

Rettig WJ, et al. Role of human chromosome 11 in determining surface antigenic phenotype of normal and malignant cells. Somatic cell genetic analysis of eight antigens, including putative human Thy-1. J. Exp. Med. 162: 1603-1619, 1985. PubMed: 2865325

Rettig WJ, et al. Differential expression of the human Thy-1 gene in rodent-human somatic cell hybrids [corrected] [published erratum appears in J. Immunol. 139: 1747, 1987]. J. Immunol. 138: 4484-4489, 1987. PubMed: 2884261

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