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J82

規(guī)格:

價格:

編號:B164880

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

J82

商品貨號

B164880

Organism

Homo sapiens, human

Tissue

urinary bladder

培養(yǎng)基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

transitional cell carcinoma

Age

58 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

The cell line is aneuploid human male (XY), with most chromosome counts in the triploid range. However, the chromosome count range is quite broad, extending from hyperdiploid to hexaploid. Normal chromosomes N11 and N20 are under-represented with respect to the other normal chromosomes: altered forms of these two chromosomes are prominent as marker chromosomes. Chromosome N13 tends towards over-representation. Five marker chromosomes are noted: 20q+, 11q+, 8p+, del(1)(q31), 5p+(HSR). One of these, 20q, is identical to marker chromosome M1 of C. O'Toole, et al., Br. J. Cancer 38: 64, 1978. The remainder are not as clearly related to the original marker chromosomes noted by those authors.

Clinical Data

male

58 years

Caucasian, Swedish

Antigen Expression

HLA A2, Aw32, B5, B12, Cw5; Blood Type A

Tumorigenic

Yes

Effects

Yes, in nude mice

Comments

Electron microscopic examination did not reveal desmosomes but varying amounts of rough endoplasmic reticulum and prominent microfilaments were observed.

Contains the ras (H-ras) oncogene.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 10,12

D16S539: 11,12

D5S818: 12,13

D7S820: 9,11

THO1: 9.3

TPOX: 11,12

vWA: 17,18

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1-2

PGM1, 1

PGM3, 2

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