最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > J5-2 B164877
J5-2
J5-2
規格:
價格:
編號:B164877
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
J5-2
商品貨號
B164877
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with endotoxins from Gram negative bacteria, and inhibits endotoxin induced mitogenesis of B cells.
The antibody is protective in mice against Pseudomonas aeruginosa burn infection.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified E. coli J5 endotoxin core.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Escherichia coli J5 endotoxin core
Cellular Products
immunoglobulin; monoclonal antibody; against Escherichia coli J5 endotoxin core
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with purified E. coli J5 endotoxin core.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with endotoxins from Gram negative bacteria, and inhibits endotoxin induced mitogenesis of B cells.
The antibody is protective in mice against Pseudomonas aeruginosa burn infection.
The epitope recognized by J5-2 appears to be distinct from that recognized by J5-1 (ATCC HB-8297).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
RPMI 1640 medium with non-essential amino acids, 1 mM sodium pyruvate and 2 mM L-glutamine, 72.5%; fetal bovine serum, 20%; DMSO, 7.5%
Isotype
IgG1
Name of Depositor
Centocor, Inc.
U.S. Patent Number
References

Pollack M, Hunter KW. Monoclonal antibodies reactive with endotoxin core. US Patent 5,057,598 dated Oct 15 1991

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479