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IL-A51
IL-A51
規格:
價格:
編號:B164853
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
IL-A51
商品貨號
B164853
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a 34000 to 35000 dalton antigen on bovine T cells that is equivalent to human CD8.
This is one of a series of hybridomas produced by Dr.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD).
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine CD8
Cellular Products
immunoglobulin; monoclonal antibody; against bovine CD8
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with bovine lymphocytes.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody reacts with a 34000 to 35000 dalton antigen on bovine T cells that is equivalent to human CD8.
This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD).
Complete Growth Medium
RPMI 1640 medium with 0.02 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
FA Osorio, WI Morrison
Deposited As
mouse (B cell); mouse (myeloma)
References

Clevers H, et al. Identification of a bovine surface antigen uniquely expressed on CD4- CD8- T cell receptor gamma/delta+ T lymphocytes. Eur. J. Immunol. 20: 809-817, 1990. PubMed: 1971793

Ellis JA, et al. Characterization by a monoclonal antibody and functional analysis of a subset of bovine T lymphocytes that express BoT8, a molecule analogous to human CD8. Immunology 58: 351-358, 1986. PubMed: 2426182

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