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當(dāng)前位置: 首頁 > ATCC代理 > IL-A11 B164849
IL-A11
IL-A11
規(guī)格:
價(jià)格:
編號:B164849
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
IL-A11
商品貨號
B164849
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a 52000 to 64000 dalton antigen on bovine T cells that is equivalent to human CD4.
This is one of a series of hybridomas produced by Dr.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD).
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine CD4
Cellular Products
immunoglobulin; monoclonal antibody; against bovine CD4
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with uncloned alloreactive bovine lymphocytes.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody reacts with a 52000 to 64000 dalton antigen on bovine T cells that is equivalent to human CD4.
This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD).
Complete Growth Medium
RPMI 1640 medium with 0.02 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
FA Osorio, WI Morrison
Deposited As
mouse (B cell); mouse (myeloma)
References

Baldwin CL, et al. Characterization of a subset of bovine T lymphocytes that express BoT4 by monoclonal antibodies and function: Similarity to lymphocytes defined by human T4 and murine L3T4. J. Immunol. 136: 4385-4391, 1986. PubMed: 2423591

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