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IgH-2

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價格:

編號:B164848

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

IgH-2

商品貨號

B164848

Organism

iguana

Tissue

heart

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

immature

Gender

male

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 35; range = 30 to 64.
The karyotype is stable. There is one large marker chromosome with a terminal centromere appearing in all cells in addition to 22 minute chromosomes, 10 large chromosomes with a median centromere and 2 large chromosomes with a submedian centromere.

Virus Susceptibility

iguana
Herpes simplex virus
Pseudorabies virus
Vaccinia virus
Human poliovirus 1
Vesicular stomatitis virus

Complete Growth Medium

Eagles's Minimum Essential Medium with Hanks BSS, 2 mM L-glutamine, and 0.1 mM non-essential amino acids supplemented with 10% fetal bovine serum

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 36°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension into new culture vessels.
Incubate cultures at 36°C in a closed flask without CO₂ in air atmosphere.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Twice per week

Cryopreservation

Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 36°C
Atmosphere: Air, 100%; no CO₂

Name of Depositor

HF Clark

Deposited As

iguana

Year of Origin

August, 1966

References

Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904

Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Cross References

Nucleotide (GenBank) : L27530 Iguana small cytoplasmic RNA (Y3).

Nucleotide (GenBank) : L27537 Iguana small cytoplasmic RNA (Y4).

Nucleotide (GenBank) : L27532 Iguana small cytoplasmic RNA (Y3) gene.

Nucleotide (GenBank) : L27534 Iguana small cytoplasmic RNA (Y4) gene.

Nucleotide (GenBank) : L27536 Iguana small cytoplasmic RNA (Y4) gene.

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