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當(dāng)前位置: 首頁 > ATCC代理 > IgG-6A6 B164844
IgG-6A6
IgG-6A6
規(guī)格:
價格:
編號:B164844
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
IgG-6A6
商品貨號
B164844
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a peptide corresponding to the 10 carboxy terminal amino acids (SVVSTDDDLA) of the rat VLDL receptor coupled to keyhole limpet hemocyanin.
It can be used in immunoblotting assays to detect VLDL receptors.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against very low density lipoprotein (VLDL) receptor
Cellular Products
immunoglobulin; monoclonal antibody; against very low density lipoprotein (VLDL) receptor
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with a peptide corresponding to the 10 carboxy terminal amino acids (SVVSTDDDLA) of the rat VLDL receptor coupled to keyhole limpet hemocyanin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes the cytoplasmic domain of the VLDL receptor from various tissues and species.
It can be used in immunoblotting assays to detect VLDL receptors.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG1
Name of Depositor
JL Goldstein, YK Ho
Deposited As
mouse (B cell); mouse (myeloma)
References

Jokinen EV, et al. Regulation of the very low density lipoprotein receptor by thyroid hormone in rat skeletal muscle. J. Biol. Chem. 269: 26411-26418, 1994. PubMed: 7929362

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