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IgG-5D7 [anti LRP 515]
IgG-5D7 [anti LRP 515]
規(guī)格:
價格:
編號:B164843
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
IgG-5D7 [anti LRP 515]
商品貨號
B164843
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts specifically with the 515 kDa subunit of rabbit LRP.
Derivation
Animals were immunized with purified rabbit LRP.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the 515000 dalton (515 kDa) subunit of rabbit lipoprotein receptor related protein (LRP)
Cellular Products
immunoglobulin; monoclonal antibody; against the 515000 dalton (515 kDa) subunit of rabbit lipoprotein receptor related protein (LRP)
Comments
Animals were immunized with purified rabbit LRP.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts specifically with the 515 kDa subunit of rabbit LRP.
It does not react with the 85 kDa subunit of rabbit LRP, nor does it cross-react with the 515 kDa subunit of rat LRP.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 1 to 2 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
YK Ho, JL Goldstein
Deposited As
mouse (B cell); mouse (myeloma)
References

Herz J, et al. Low density lipoprotein receptor-related protein mediates endocytosis of monoclonal antibodies in cultured cells and rabbit liver. J. Biol. Chem. 265: 21355-21362, 1990. PubMed: 2250029

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