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IgG-3B2
IgG-3B2
規格:
價格:
編號:B164841
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
IgG-3B2
商品貨號
B164841
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a bacterially produced fusion protein expressing pGST-dSREBP (amino acids 1 to 451).
It can be used in immunoblotting assays to detect Drosophila SREBP and to study SREBP in S2 cells grown in various conditions.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Animals were immunized with a bacterially produced fusion protein expressing pGST-dSREBP (amino acids 1 to 451).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Drosophila sterol regulatory element binding protein (SREBP)
Cellular Products
immunoglobulin; monoclonal antibody; against Drosophila sterol regulatory element binding protein (SREBP)
Comments
Animals were immunized with a bacterially produced fusion protein expressing pGST-dSREBP (amino acids 1 to 451).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes both the precursor and mature forms of the Drosophila SREBP transcription factor in cultured S2 (Schneider) Drosophila cells.
It can be used in immunoblotting assays to detect Drosophila SREBP and to study SREBP in S2 cells grown in various conditions.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
mouse IgG1 kappa
Name of Depositor
YK Ho, JL Goldstein
Deposited As
mouse (B cell); mouse (myeloma)
References

Seegmiller AC, et al. The SREBP pathway in Drosophila: regulation by palmitate, not sterols. Dev. Cell 2: 229-238, 2002. PubMed: 11832248

Dobrosotskaya IY, et al. Regulation of SREBP processing and membrane lipid production by phospholipids in Drosophila.. Science 296: 879-883, 2002. PubMed: 11988566

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