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IgG-11H9
IgG-11H9
規格:
價格:
編號:B164837
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
IgG-11H9
商品貨號
B164837
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a bacterially produced fusion protein of Shistosoma japonicum glutathione S-transferase and rabbit OSBP (the fusion protein was cleaved, and the glutathione S-transferase removed before immunization).
The antibody is useful for studies of localization and distribution of OSBP in cultured cells, as well as for tracking movement of OSBP after cell perturbation.
Derivation
Animals were immunized with a bacterially produced fusion protein of Shistosoma japonicum glutathione S-transferase and rabbit OSBP (the fusion protein was cleaved, and the glutathione S-transferase removed before immunization).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rabbit oxysterol binding protein (OSBP)
Cellular Products
immunoglobulin; monoclonal antibody; against rabbit oxysterol binding protein (OSBP)
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with a bacterially produced fusion protein of Shistosoma japonicum glutathione S-transferase and rabbit OSBP (the fusion protein was cleaved, and the glutathione S-transferase removed before immunization).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes OSBP in mammalian tissues and cultured cells.
The antibody is useful for studies of localization and distribution of OSBP in cultured cells, as well as for tracking movement of OSBP after cell perturbation.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG1
Name of Depositor
JL Goldstein, YK Ho
Deposited As
mouse (B cell); mouse (myeloma)
References

Ridgway ND, et al. Translocation of oxysterol binding protein to Golgi apparatus triggered by ligand binding. J. Cell Biol. 116: 307-319, 1992. PubMed: 1730758

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