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Ig(1a)8.3 (formerly 20-8.3)
Ig(1a)8.3 (formerly 20-8.3)
規格:
價格:
編號:B164831
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Ig(1a)8.3 (formerly 20-8.3)
商品貨號
B164831
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the Fc fragment, not the Fab fragment of mouse IgG2a, at a determinant probably located within the CH2 region.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the Ig-1a allotype on mouse IgG2a
Cellular Products
immunoglobulin; monoclonal antibody; against the Ig-1a allotype on mouse IgG2a
Comments
Animals were immunized with antigen - antibody complexes (BALB/c anti Bordetella pertussis with B pertussis).
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with the Fc fragment, not the Fab fragment of mouse IgG2a, at a determinant probably located within the CH2 region.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 20%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
    Isotype
    IgG2a
    Name of Depositor
    LA Herzenberg
    Deposited As
    mouse (B cell); mouse (myeloma)
    References

    Oi VT, Herzenberg LA. Localization of murine Ig-1b and Ig-1a (IgG2a) allotypic determinants detected with monoclonal antibodies. Mol. Immunol. 16: 1005-1017, 1979. PubMed: 94425

    Oi VT, et al. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr. Top. Microbiol. Immunol. 81: 115-129, 1978. PubMed: 567555

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