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IFGCP-F1BA10
IFGCP-F1BA10
規(guī)格:
價(jià)格:
編號:B164830
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
IFGCP-F1BA10
商品貨號
B164830
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human gamma interferon (IFN)
Cellular Products
immunoglobulin; monoclonal antibody; against human gamma interferon (IFN)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with a synthetic polypeptide 19 residues long (NH2-Lys-Thr-Gly-Lys-Arg-Lys-Arg-Ser-Gln-Met-Leu-Phe-Arg-Gly-Arg-Arg-Ala-Ser-Gln-COOH) bound to KLH.
Spleen cells were fused with Sp2/0 myeloma cells.
Complete Growth Medium
HB101 medium (Hana Biologicals), 99%; fetal bovine serum, 1% - OR - Dulbecco's modified Eagle's medium, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 cells/ml and maintain at or below 8 X 10 exp5 cells/ml.
Cryopreservation
fetal bovine serum, 90%; DMSO, 10%
Isotype
IgG1
Name of Depositor
Amgen, Inc.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Altrock BW. Monoclonal antibodies which specifically bind to human immune interferon. US Patent 4,599,306 dated Jul 8 1986

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