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I1-Hybridoma

規格:

價格:

編號:B164820

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

I1-Hybridoma

商品貨號

B164820

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Tissue

spleen

培養基

Cell Type

hybridoma: B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Vesicular Stomatitis

Age

adult

Applications

The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind.

Storage Conditions

liquid nitrogen vapor phase

Derivation

Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.

Tumorigenic

Yes

Effects

Yes, intraperitoneal injection into BALB/c or (BALB/c X BALB.B)F1 mice

Comments

Animals were immunized with vesicular stomatitis virus, Indiana Serotype (VSV-Ind.).

Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.

The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind.).

Complete Growth Medium

Minimum essential medium (Eagle) with 2.0 mM L-glutamine and Hanks' BSS adjusted to contain 0.35 g/L sodium bicarbonate 80%; fetal bovine serum, 20%.

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Note: This medium is formulated for use in a free gas exchange with atmospheric air. A 5% CO2 in air atmosphere is detrimental to cells when using this medium for culturing.

Cryopreservation

Freeze medium: Complete growth medium supplemented with an additional 45% fetal bovine serum and 10% DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: Air, 100%

Isotype

mouse IgG2a kappa

Name of Depositor

IS Novella, DS Lyles

Deposited As

Mus musculus (B cell); Mus musculus (myeloma)

References

Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982. PubMed: 6180550

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