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Hs 766T

規格:

價格:

編號:B164736

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Hs 766T

商品貨號

B164736

Organism

Homo sapiens, human

Tissue

pancreas; derived from metastatic site: lymph node

培養基

Cell Type

Epithelial

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

pancreatic carcinoma

Age

64 years

Gender

male

Ethnicity

Caucasian

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor temperature

Karyotype

(P11) hyperdiploid with mode = 60; banded markers; lacking Y chromosomes

Derivation

The Hs 766T line was isolated by R. Owens et al. at the Naval Biosciences Laboratory in 1973 from a pancreatic carcinoma metastatic to a lymph node.

Derived from metastatic site, lymph node.

Tests conducted at an early passage indicated that the cells exhibited many properties observed only in tumor lines.

Clinical Data

46 years

Caucasian

male

Tumorigenic

Yes

Effects

Yes, in immunosuppressed mice

Comments

The epithelial nature of the cell strain selected was confirmed by electron microscopy as microvilli and desmosomes were observed.

Tests conducted at an early passage indicated that the cells exhibited many properties observed only in tumor lines. The karyotype was abnormal and the cells formed colonies in Methocel and on contact inhibited monolayers.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

STR Profile

Amelogenin: X

CSF1PO: 12

D13S317: 8,11

D16S539: 9,12

D5S818: 11

D7S820: 10

THO1: 6,9.3

TPOX: 8

vWA: 18

Isoenzymes

AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1-2

PGM3, 1

Name of Depositor

RB Owens

Deposited As

Homo sapiens

Year of Origin

1973

References

Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412

Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

. Prevention and detection of cancer. vol. 2New York: Marcel Dekker; 1978.

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