Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
normal
Age
newborn
Gender
male
Ethnicity
Caucasian
Karyotype
diploid
Derivation
Hs 67 was developed in 1969 at the Naval Biosciences Laboratory by R. Owens from the thymus of a 6 pound, apparently normal, male.
Clinical Data
Hs 67 was developed in 1969 at the Naval Biosciences Laboratory by R. Owens from the thymus of a 6 pound, apparently normal, male.
male
Caucasian
newborn
Comments
This line was also previously distributed by ATCC as CRL-7828 (Hs 67.Th).
Hs 67 was developed in 1969 at the Naval Biosciences Laboratory by R. Owens from the thymus of a 6 pound, apparently normal, male.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with Hank's Balanced Salt Solution or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
