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HPAC

規(guī)格:

價格:

編號:B164644

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

HPAC

商品貨號

B164644

Organism

Homo sapiens, human

Tissue

pancreas

培養(yǎng)基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

adenocarcinoma

Age

64 years

Gender

female

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 61

Derivation

HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin.

Clinical Data

64 years

female

Caucasian

Receptor Expression

epidermal growth factor (EGF), expressed

glucocorticoid, expressed

epidermal growth factor (EGF); glucocorticoid

Genes Expressed

HPAC cells are positive for keratin and negative for vimentin and chromogranin A.

Tumorigenic

Yes

Effects

Yes, the cells form tumors in athymic nude mice at the site of inoculation which are histologically similar to the tumor of origin

Yes, the cells have a colony forming efficiency of 64% on plastic and 3.2% in agarose.

Comments

HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha).

Cell growth is suppressed by dexamethasone and other glucocorticoids.

Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9.

In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells.

HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor.

Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1.

Complete Growth Medium

A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 0.002 mg/ml insulin, 0.005 mg/ml transferrin, 40 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor and 5% fetal bovine serum

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C

Subculture Ratio: 1:3 to 1:6, every 6 to 8 days.

Medium Renewal: 2 to 3 times a week.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO₂), 5%

STR Profile

Amelogenin: X

CSF1PO: 13

D13S317: 11

D16S539: 9,10

D5S818: 12

D7S820: 10,12

THO1: 9.3

TPOX: 10,11

vWA: 15,17

Population Doubling Time

41 hrs

Name of Depositor

WR Gower

Deposited As

Homo sapiens

Year of Origin

1985

References

Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994.

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