Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not react with human Fab or F(ab')2 nor does it react with human IgA, IgE or IgM.
Tested and found negative for ectromelia virus (mousepox).
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, (ATCC 30-2002). To make the complete growth medium, add the following components to the base medium:
Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201
Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923
