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HO-2.2
HO-2.2
規格:
價格:
編號:B164624
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HO-2.2
商品貨號
B164624
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The Lyt-2.2 antigen is present on mouse cytotoxic and suppressor T cells and is equivalent to the Lyt-2b serologic specificity.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Lyt-2.2 (mouse T cell antigen); cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against Lyt-2.2 (mouse T cell antigen); cytotoxic
Comments
Animals were immunized with C57BL/6 thymocytes.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The Lyt-2.2 antigen is present on mouse cytotoxic and suppressor T cells and is equivalent to the Lyt-2b serologic specificity.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
P Gottlieb
Deposited As
mouse (B cell); mouse (myeloma)
References

Gottlieb PD, et al. Construction and properties of new Lyt-congenic strains and anti-Lyt-2.2 and anti-Lyt-3.1 monoclonal antibodies. Immunogenetics 10: 545-555, 1980.

Raulet DH, et al. Fractionation of lymphocyte populations with monoclonal antibodies specific for Lyt-2.2 and Lyt-3.1. J. Immunol. 125: 1136-1143, 1980. PubMed: 6447723

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