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hIL-15-M111
hIL-15-M111
規格:
價格:
編號:B164613
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
hIL-15-M111
商品貨號
B164613
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
Animals were immunized with yeast-derived human interleukin-15 (IL-15).
The antibody reacts with IL-15, an epithelium derived T cell growth factor.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with yeast-derived human interleukin-15 (IL-15).
Spleen cells were fused with AGX653 mouse myeloma cells.
The antibody reacts with IL-15, an epithelium derived T cell growth factor.
Genes Expressed
immunoglobulin; monoclonal antibody; against interleukin 15 (IL-15)
Cellular Products
immunoglobulin; monoclonal antibody; against interleukin 15 (IL-15)
Comments
Animals were immunized with yeast-derived human interleukin-15 (IL-15).
Spleen cells were fused with AGX653 mouse myeloma cells.
The antibody reacts with IL-15, an epithelium derived T cell growth factor.
Monoclonal antibodies generated can be assayed for IL-15 antagonist activity using the CTLL-2 assay as essentially described by Gillis, et al.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp4 and 1 X 10 exp6 viable cells/ml.
Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Immunex Corporation
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Gillis S, Smith KA. Long term culture of tumour-specific cytotoxic T cells. Nature 268: 154-156, 1977. PubMed: 145543

Grabstein KH, et al. Antagonists of interleukin-15. US Patent 5,795,966 dated Aug 18 1998

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